Product information

Antibody name: Chicken anti-RbcL global (anti-Rubisco large subunit antibody)

Product number: AS01-017; Quantity: 50 ul; FAQ about anti-RbcL antibodies

Actual Price of anti-RbcL antibody: 340 $(US) or 255 EUR

PRICES INCLUDE DELIVERY AT ROOM TEMPERATURE BY EXPRESS MAIL

Immunogen: The antibody was raised against a peptide target conserved in all known RbcL proteins (Rubisco large subunit, Rubisco Form I).

Rubisco antibody will recognize denatured Rubisco large subunit from following taxonomic groups: Check here!

Type of antibody: Polyclonal; Antibody format:Total IgY fraction (around 80 % purity) in PBS;

Secondary antibody: sample of a rabbit anti-IgY HRP labelled antibodies included in the shipment.

Tested applications: Western Blot.

Detection conditions:
Primary antibody: RbcL Global Antibody 1: 50 000;
Secondary antibody: AbCam HRP conjugated rabbit anti-IgY, dilution 1:50 000
Detection: ECL Advance (AmershamBiosciences)

Anti-RbcL antibodies have been tested on whole protein extracts from following samples: mixed phytoplankton (dominated by diatoms), Lichen: Lobaria pulmonaria ; Hydrilla verticulata,
Higher plants: Medicago sativa L(alfa alfa), Maize leafs, Solanum tuberosum(potato), Pisum sativum (pea), Camelia sinensis (tea), Ulmus (american elm); Grass: Spartina alterniflora.

Protocols.
Example of Western Blot protocol with anti-RbcL antibody
Protocol for extraction of proteins from phytoplankton

Expected molecular weight of RbcL mature chain (slightly longer precursors synthesized) on SDS PAGE:

Sensitivity of anti-RbcL antibodies Sensitivity of anti-RbcL antibodies: showed by detection of different amounts of Rubisco protein.
Line 1 - MagicMarks markers (Invitrogen);
Line 2 - 0.6 ng (11.4 fmoles);
Line 3 - 3. 1.8 ng (34.2 fmoles);
Line 4 - 5.4 ng (102.6 fmoles);
Line 5 - 16.2 ng rbcL (307.8 fmoles);
Line 6 - 48.6 ng (923.4 fmoles);
Line 7 - 145.8 ng (2.77 pmoles);
Line 8 - 437.4 ng rbcL (8.31 pmoles);
Line 9 - blank;
Line 10 - extract of Synechococcus PCC 7942;
Line 11 - extract of spinach;
Line 12 - MagicMarks markers (Invitrogen);
Conclusion:Anti-RbcL antibodies can detect Rubisco protein from 102.6 fmoles, when using ECL Advance (AmershamBiosciences) as developing reagent. Both, primary antibodies and secondary antibodies were diluted to 1:50 000.

Immunolocalization: so far in a grass species on tissue which was formaldehyde-fixed and paraffin-embedded. Following the protocol from Gonzalez et al.Plant Physiol. V. 116, April 1998.

Recommended dilution: from 1: 2 000 to 1:20 000 with standard ECL.
1: 50 000 with ECL Advance.

References presenting work where global antibodies have been used: