Anti-RbcL chicken antibody is originally designed to work in Western Blot technique and detect denatured Rubisco protein. It has also been tested in immunohistochemistry and works well on tissue sections where after fixation step Rubisco protein is in the unfolded state. This is needed, since peptide sequence used to produce this antibody is derived from the inside of Rubisco protein.
NO. The antibody will not recognize a native Rubisco protein and therefore can not be used in following techniques: immunoprecipitation or sample cleaning from excess of native Rubisco protein.
NO. It is not designed to work with native Rubisco protein, therefore anti-RbcL antibody can not be applied in those techniques. AgriSera is currently working on development of antibody against native Rubisco protein from higher plants, which could be used for immunoprecipitation and sample cleaning. This antibody might be available in 2005.
Yes. Anti-RbcL antibodies will detect RbcL protein from a range of samples from higher plants, including: Arabidopsis, Medicago sativa L (alfa alfa), Maize leafs, Solanum tuberosum(potato), Pisum sativum (pea), Camelia sinensis (tea), Ulmus (american elm) and many others.
Anti-RbcL antibodies have been so far tested on following samples:
Cyanobacteria: Synechococcus sp. PCC 7942, Synechocystis 6804;
Red algae;
Mixed phytoplankton (dominated by diatoms);
Lichen: Lobaria pulmonaria ; Hydrilla verticulata;
Higher plants: Medicago sativa L(alfa alfa), Maize leafs, Solanum tuberosum(potato), Pisum sativum (pea), Camelia sinensis (tea), Ulmus (american elm);
Grass: Spartina alterniflora;
For a complete list of species antibodies will cross-react with:
Please, check here!
Example of Western Blot protocol with anti-RbcL antibody
Protocol for extraction of
proteins from phytoplankton
Following secondary antibodies have been used in testing:
Sigma anti chicken IgY, HRP conjugated, product number: A9046.
Abcam anti-chicken IgY, HRP conjugated, product number: ab6753.
Detection system: for example ECL Advance (Amersham Biosciences).
Used dilution has to be adjusted, depending upon used system.
Sample of a secondary antibody anti-IgY, HRP conjugated from Sigma,
will be shipped with every ordered global antibody sample.
Anti-RbcL antibodies in the form of total IgY solution with sodium azide at 0.02 % will
be delivered by express mail at room temperature, together with a secondary antibody
sample HRP labelled.
Such shipment conditions have been tested for this antibody. Delivery time is around one week.
By a bank transfer to AgriSera account, using SWIFT/IBAN.
No credit card payment.
Customers from EU countries should provide VAT number for billing purpose.
Customers from US and Canada should provide a purchase order number.
Customers from other parts of the world: pre-payment is required.
Antibodies have to be ordered directly from AgriSera via e-mail, homepage, or fax.
For customers from Japan: there are two distributors: Funakoshi and CosmoBio.
There is no specific MSDS data sheet for this antibody. All information required will be found on the product information sheet which is sent together with the antibody.