Secondary antibodies conjugated to HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase) enzymes are most frequently used to visualize target proteins in Western blot, ELISA and immunolocalization. When using secondary antibodies conjugated to HRP, the detection is performed using chemiluminescence (recorded by a CCD camera or another imager), which gives high sensitivity and good resolution in both Western blot and chemiluminescent ELISA. When using antibodies conjugated to ALP, visualization of the antibody binding to the target is achieved using chromogenic reagents, and no CCD camera is necessary. Agrisera's secondary antibody collection is composed of over 220 antibodies; both highly purified and available in a basic format, suitable for Western blot, as well as adsorbed to serum from a wide range of animal species, suitable for immunolocalization and ELISA. Based on the analysis of antibody performance in available publications, conducted by CiteAB, it became very clear that Agrisera secondary antibodies are performing above market average. This means that Agrisera secondary antibodies conjugated to ALP or HRP can be used at high dilutions and are price-worthy reagents, and will provide you with a consistent performance over many experiments.
| We are always eager to provide prompt advice on which Agrisera secondary antibody is best suited for the experiments you aim to perform. Contact us at [email protected]! |
Latest
Agrisera antibodies to photosynthetic proteins support research on how to improve cop yields2024-06-08 Agrisera supports two conferences this week
2024-06-07 Meet with Agrisera at 2nd American Photosynthesis Congress
2024-06-04 Agrisera Western blot workshop for North America
2024-05-31 Agrisera supports 13th International Coference for Plant Mitochondrial Biology
2024-05-27 Study chloroplast biogenesis and architecture with Agrisera antibodies
2024-05-24 Investigate proteins involved in sucrose metabolism with Agrisera antibodies
2024-05-19 Agrisera supports 9th Plant Biochemistry Symposium: Plant Metabolites and Signaling
2024-05-17 Track changes in LHC proteins using most published anti-LHC antibodies
2024-05-12 Agrisera supports BioLock student conference at the University of Wrocław
2024-05-10
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